PCR PROTOCOLS IN THE DETECTION OF THE SECOND EXON OF THE BoLA-DRB3.2 GENE

Abstract

The bovine leukocyte antigen (BoLA) gene, plays an important role in the immune response and has been associated with the resistance or susceptibility of infectious and autoimmune diseases such as mastitis, leucosis, dermatophilosis, internal and external parasites, responsible for significant economic losses. There are groups of authors that have performed molecular studies and apply PCR protocols where the BoLA-DRB3.2 gene is amplified. The objective of this work was to evaluate the amplification protocols of the second exon of the BoLA-DRB3.2 gene in cattle developed by Dietz et al. (A) and Takeshima et al. (B). A random sampling of hair from the caudal area of 50 cattle was carried out in several sites of the Republic of Panama. Considering the results observed in the present study, protocol B achieved the best amplifications of the second exon of the BoLA-DRB3.2 gene, being the one of choice to deepen the follow up of the investigations that involve these gene in Panamanian cattle.